Baltimore — At the 73rd Annual Conference on Mass Spectrometry and Allied Topics (ASMS), Bruker Corporation unveiled the proteoElute nanoLC system and next-generation PepSep Advanced nLC columns, delivering a major leap in sensitivity, robustness, and throughput for ultra-sensitive proteomics workflows. These new technologies are designed to meet the growing demands of cutting-edge applications like single-cell proteomics, immunopeptidomics, and microbiome metaproteomics profiling.
The proteoElute nanoLC system is engineered with biocompatible ceramic valves, proteoTrap trapping columns, and Bruker’s new TwinScape™ real-time diagnostic technology. This integrated system offers improved durability, reduced carry-over, and seamless performance even with low-input or highly complex biological samples. TwinScape continuously monitors instrument health and alerts users to potential issues before they affect experimental outcomes—an especially valuable feature when working with rare or irreplaceable samples.
The system’s robust architecture also includes a new injector and needle wash system that reduces carry-over by up to threefold, ensuring cleaner runs and greater confidence in low-abundance peptide detection. Designed with reliability in mind, the proteoElute platform allows researchers to perform advanced proteomics workflows at scale with minimal downtime.
Supporting the new system is Bruker’s PepSep Advanced column line, designed to deliver sharper chromatographic peaks and higher resolution separations. These columns showed up to 20% gains in peptide identifications and 10% increases in protein identifications in benchmark tests. When used in combination with Bruker’s CaptiveSpray Ionization (CSI) emitters, the PepSep columns offer extended lifespans, easy maintenance, and lower long-term operating costs.
Researchers are already seeing the impact. W. Hayes McDonald of Vanderbilt University said the combined platform provides the flexibility and performance needed for today’s most demanding proteomic studies. At the Children’s Hospital of Philadelphia (CHOP-Penn) Proteomic Core Facility, Technical Director Lynn Spruce noted that the new PepSep columns deliver highly reproducible results with improved peak symmetry and no tailing, describing the system as a “plug-and-play” solution.
With the introduction of proteoElute and the new PepSep Advanced columns, Bruker is positioning itself at the forefront of high-sensitivity proteomics, enabling researchers to push the boundaries of discovery across biological and clinical research.
